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Suzuki, Shinichi; Yaita, Tsuyoshi; Sugo, Yumi; Kimura, Takaumi
no journal, ,
no abstracts in English
Matsumura, Tatsuro; Takeshita, Kenji*; Mori, Atsunori*
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no abstracts in English
Shimojo, Kojiro; Oshima, Tatsuya*; Naganawa, Hirochika; Goto, Masahiro*
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We have found that a calix[6]arene acetic acid derivative forms a supramolecular complex with urea-denatured cytochrome c at the oil-water interface, which enables quantitative transfer of the protein from an 8 M urea aqueous solution into an organic phase through a proton-exchange mechanism. Denatured cytochrome c is completely separated from the denaturant, and is isolated from other denatured cytochrome c molecules to suppress the generation of aggregates due to protein-protein interactions. The recovery of cytochrome c from the organic phase is successfully achieved under acidic conditions using an appropriate amount of 1-butanol. UV-visible, CD, and fluorescence spectroscopic characterizations demonstrate that cytochrome c transferred into a denaturant-free aqueous solution regains its native structure.
Nakatsuru, Kano; Shimojo, Kojiro; Naganawa, Hirochika; Tsukahara, Satoshi*; Fujiwara, Terufumi*
no journal, ,
no abstracts in English
Kurahashi, Kensuke; Shimojo, Kojiro; Naganawa, Hirochika; Umetani, Shigeo*; Sorin, Yoshiki*
no journal, ,
no abstracts in English